Finding antigen negative blood is difficult and time consuming, especially when using serologic methods. The Blood Center of Wisconsin recently implemented mass-scale red blood cell genotyping on non-Caucasian donors and repeat donors who had a recent blood donation.
Over four years, 43,000 donors were screened for 32 single nucleotide polymorphisms that predicted 42 blood group antigens. There was a discordance rate of 0.52% between real-time PCR mass-scale genotyping and serologic phenotypes. Since mass-scale genotyping is accurate, has a rapid turn-around time and can handle more than 700 samples per day, this large blood center was able to abandon serologic screening.
- Flegel WA, Gottschall JL, Denomme GA. Implementing mass-scale red cell genotyping at a blood center. Transfusion 2015; Epub ahead of print. doi: 10.1111/trf.13168.
Transfusion offers CME credit for this study! Log on at www.wileyhealthlearning.com/trf.